opuBD [2018-12-18 17:47:18]

choline and arsenocholine [SW|ABC transporter] (membrane protein)

locus: BSU33700

pI: 10.06

mw: 23.78 kDa

protein length: 226 aa

gene length: 678 bp

function: compatible solute transport

product: choline and arsenocholine [SW|ABC transporter] (membrane protein)

essential: no

synonyms: proZ

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,459,806 → 3,460,486

The protein

Catalyzed reaction/ biological activity

• uptake of choline and arsenocholine [pubmed|29159878]

Protein family

• CysTW subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|76C8D6BF2CA1FA5E7392C52FFD55FD01EF5AB9DC|OpuCD]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]
  description: [Pubmed|10216873]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10216873], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon]
  • [protein|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR]: repression, (sterical interference with RNA polymerase access) [Pubmed|32849357,23960087], in [regulon|9F32E96AC9C32832C2F10FB125DDDC50EC9B546B|OpcR regulon]
  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

  regulation

  • induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163]
  • expression increases with increasing salinity [Pubmed|32849357]

  additional information

  • [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] acts as [SW|ncRNA|antisense RNA] for the [gene|3F502A4DCB1DAD7C3F968465B13C35213240515B|opuBA]-[gene|70B45E7428DB1F6AEF7D2FE3062CC86BE96674FC|opuBB]-[gene|C48798FE13B1521236F5BC5786B9A02D7BC9A336|opuBC]-[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD] operon [PubMed|,32373088,20525796]
  • expression starts with a delay after osmotic upshift due to transcriptional interference of [SW|RNA polymerase] complexes driving synthesis of the converging opuB and [gene|6FCF6F71C5A8B2345F42BD0C3345C520FD40358B|S1290] mRNAs [pubmed|32373088]

Biological materials

Mutant

• BKE33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG
• BKK33700 (Δ[gene|1532EC3D741C5AB3D0B642741218FAE00AD460FA|opuBD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCATTGACCTCTTCC, downstream forward: _UP4_TAGAATAGAAAAAGAGGCTG

References

Reviews

• 27935846

Original publications

• 10092453,9925583,10216873,21296969,20525796,22408163,23960087,23646920,29159878